Composite

Part:BBa_K228865:Design

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-09-22)


pSal-rbs-PhiR73 ( amber mutation )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.


Source

part registery 2009 distribution & PKU 2009 igem team

References